Ai! Lá polin saca i quettar!
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A preposition for “from”, especially in the genitival sense “originating from”. For “from” in a positional sense, it is far more common to use the ablative suffix -llo.
Conceptual Development: The preposition ᴱQ. ô was first mentioned in the Gnomish Lexicon of the 1910s as the equivalent of G. a(n·) “from” which had an ablative sense (GL/17). In Primitive Quendian Structure: Final Consonants written in 1936, Tolkien mentioned {o >> ho >>} o as a preposition based on primitive ᴹ✶ʒō̆ “away from, from among” (PE21/60 and note #48). In The Etymologies of the 1930s, ᴹQ. ho “from” appeared under the root ᴹ√ƷŌ̆ “from, away, from among, out of” (Ety/ƷŌ̆). This primitive form ʒō̆ was also the basis of the Quenya genitive suffix ᴹQ. -o.
In Definitive Linguistic Notes (DLN) from 1959, Tolkien mentioned the preposition Q. o “from” as a reduction of ancient ✶ăwă “away” (PE17/148). In Late Notes on Verb Structure (LVS) written in 1969 Tolkien again mentioned ō < ✶hō “from” with some difficult-to-read qualifications that seem to indicate this was “from” in the genitival sense, as opposed to ✶lō “from” in the positional/ablative sense (PE22/168).
Neo-Quenya: For purposes of Neo-Quenya I would assume that o is a rarely used preposition, usually replaced by either genitive -o [originating from] or ablative -llo [moving from].